Rabbit Anti-Hypusine Recombinant Antibody (PABL-202)

Customer Review

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Laura Hernandez
05.Feb,23

Ideal for Flow Cytometry

The Anti-Hypusine Recombinant Antibody (clone Hpu24) has proven to be incredibly reliable in our flow cytometry and immunofluorescence applications. The results are always high-quality and reproducible, with strong affinity and specificity for Hypusine. It has become a staple in our lab for various experiments, providing us with the confidence to proceed with complex assays. The robust performance of this antibody has significantly enhanced the quality of our data.

Brian Anderson
14.Nov,21

Great for Western Blots

We incorporated this antibody into our Western blot assays, and it has been fantastic. The specificity and sensitivity for Hypusine are impressive, consistently delivering clear and precise results. It has made our research process smoother and more reliable.

Megan Thompson
22.Jan,24

Reliable in Multiple Applications

This antibody has been reliable across various applications, including flow cytometry and immunofluorescence. The high-quality, reproducible results and strong affinity for Hypusine have significantly benefited our experiments, making it an indispensable tool in our lab. The consistent performance has greatly enhanced our data quality.

Q&As

1. Is the anti-Hypusine recombinant antibody clone Hpu24 specific for eIF5A?

   A: Yes, the anti-Hypusine recombinant antibody clone Hpu24 (PABL-202) is specific for eIF5A. It has been validated to detect hypusine-modified eIF5A, making it a reliable tool for studying the role of this protein in various biological processes.

2. What applications are suitable for using the anti-Hypusine recombinant antibody clone Hpu24?

   A: The anti-Hypusine recombinant antibody clone Hpu24 (PABL-202) is suitable for applications such as Western blotting, immunoprecipitation, and immunofluorescence. It provides specific detection of hypusine-modified eIF5A in these types of experiments.

3. Can the anti-Hypusine recombinant antibody clone Hpu24 be used for flow cytometry analysis?

   A: Yes, the anti-Hypusine recombinant antibody clone Hpu24 (PABL-202) can be used for flow cytometry applications. It is specifically designed to bind to hypusine-modified eIF5A, making it suitable for such use.

4. What is the optimal dilution for using the anti-Hypusine recombinant antibody clone Hpu24 in immunofluorescence?

   A: The optimal dilution for using the anti-Hypusine recombinant antibody clone Hpu24 (PABL-202) in immunofluorescence is typically 1:100 to 1:500. It is advisable to perform a dilution series to determine the best working concentration for specific experimental conditions.

5. Can the anti-Hypusine recombinant antibody clone Hpu24 be used in immunohistochemistry on formalin-fixed, paraffin-embedded (FFPE) tissue sections?

   A: Yes, the anti-Hypusine recombinant antibody clone Hpu24 (PABL-202) can be used in immunohistochemistry on FFPE tissue sections. It provides specific staining of hypusine-modified eIF5A, allowing for detailed analysis of its expression in tissue samples.

View the frequently asked questions answered by Creative Biolabs Support.

Citations

1. Tan, Thomas CJ, et al. "Translation factor eIF5a is essential for IFNγ production and cell cycle regulation in primary CD8+ T lymphocytes." Nature Communications 13.1 (2022): 7796. https://doi.org/s41467-022-35252-y
   This study investigates the essential role of the translation factor eIF5a in the production of interferon-gamma (IFNγ) and the regulation of the cell cycle in primary CD8+ T lymphocytes. The research demonstrates that eIF5a is dynamically regulated through post-translational modification in naïve CD8+ T cells upon activation, facilitating the translation of specific subsets of proteins crucial for long-term survival and effector functions. The study underscores the importance of eIF5a in the coordination of immune responses by controlling the translation of mRNAs that regulate proliferation and key effector functions, including the production of IFNγ.
   At Creative Biolabs, we supplied the hypusine antibody Hpu24 (Cat#: PABL-202) used in flow cytometry and Western blotting analyses. Our products were crucial in enabling the detailed examination of eIF5a's role in T cell activation and function. This study's significance lies in its potential to uncover new therapeutic targets for enhancing immune responses and treating conditions related to immune dysfunction, highlighting our commitment to advancing immunological research and innovative solutions.
2. Zhang, Hanlin, et al. "Polyamines control eIF5A hypusination, TFEB translation, and autophagy to reverse B cell senescence." Molecular cell 76.1 (2019): 110-125. https://doi.org/10.1016/j.molcel.2019.08.005
   This study reveals a significant pathway in immune aging, showing how the polyamine metabolite spermidine controls cellular autophagy through a mechanism involving eIF5A hypusination and TFEB translation. The researchers demonstrate that spermidine levels decline with age, leading to reduced eIF5A hypusination, decreased TFEB expression, and impaired autophagy in immune cells, particularly B cells. Remarkably, supplementing spermidine restored this pathway and improved B cell responses in both aged mice and humans, effectively rejuvenating immune function. The study identifies a novel translational control mechanism for autophagy regulation that can potentially be targeted to reverse immune senescence.
   Creative Biolabs provided the hypusine-specific antibody (PABL-202) to detect and quantify hypusinated eIF5A in various cell types and experimental conditions. This antibody allowed for precise monitoring of the post-translational modification status of eIF5A, which was essential for establishing the mechanistic link between spermidine levels, eIF5A hypusination, TFEB expression, and autophagy activity. The ability to specifically track hypusinated eIF5A was fundamental to demonstrating how this pathway becomes dysregulated during aging and how it can be restored through spermidine supplementation.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# PABL-202, RRID: AB_2877637)

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Publications

Our products have been used extensively by scientists all over the world to accelerate their life science research. Here, we present a collection of publications that cite this product.

Zhang, Hanlin, et al. "Polyamines control eIF5A hypusination, TFEB translation, and autophagy to reverse B cell senescence." Molecular cell 76.1 (2019): 110-125.

Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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