Rabbit Anti-Hypusine Recombinant Antibody (PABL-202) (CAT#: PABL-202)

Tan, Thomas CJ, et al. "Translation factor eIF5a is essential for IFNγ production and cell cycle regulation in primary CD8+ T lymphocytes." Nature Communications 13.1 (2022): 7796. https://doi.org/s41467-022-35252-y

This study investigates the essential role of the translation factor eIF5a in the production of interferon-gamma (IFNγ) and the regulation of the cell cycle in primary CD8+ T lymphocytes. The research demonstrates that eIF5a is dynamically regulated through post-translational modification in naïve CD8+ T cells upon activation, facilitating the translation of specific subsets of proteins crucial for long-term survival and effector functions. The study underscores the importance of eIF5a in the coordination of immune responses by controlling the translation of mRNAs that regulate proliferation and key effector functions, including the production of IFNγ.
At Creative Biolabs, we supplied the hypusine antibody Hpu24 (Cat#: PABL-202) used in flow cytometry and Western blotting analyses. Our products were crucial in enabling the detailed examination of eIF5a's role in T cell activation and function. This study's significance lies in its potential to uncover new therapeutic targets for enhancing immune responses and treating conditions related to immune dysfunction, highlighting our commitment to advancing immunological research and innovative solutions.

Zhang, Hanlin, et al. "Polyamines control eIF5A hypusination, TFEB translation, and autophagy to reverse B cell senescence." Molecular cell 76.1 (2019): 110-125. https://doi.org/10.1016/j.molcel.2019.08.005

This study reveals a significant pathway in immune aging, showing how the polyamine metabolite spermidine controls cellular autophagy through a mechanism involving eIF5A hypusination and TFEB translation. The researchers demonstrate that spermidine levels decline with age, leading to reduced eIF5A hypusination, decreased TFEB expression, and impaired autophagy in immune cells, particularly B cells. Remarkably, supplementing spermidine restored this pathway and improved B cell responses in both aged mice and humans, effectively rejuvenating immune function. The study identifies a novel translational control mechanism for autophagy regulation that can potentially be targeted to reverse immune senescence.
Creative Biolabs provided the hypusine-specific antibody (PABL-202) to detect and quantify hypusinated eIF5A in various cell types and experimental conditions. This antibody allowed for precise monitoring of the post-translational modification status of eIF5A, which was essential for establishing the mechanistic link between spermidine levels, eIF5A hypusination, TFEB expression, and autophagy activity. The ability to specifically track hypusinated eIF5A was fundamental to demonstrating how this pathway becomes dysregulated during aging and how it can be restored through spermidine supplementation.